《植物生理学报》 2015, 51(10): 1617-1624

水稻核心种质的耐镉性鉴定

江淼, 余海娟, 李亮, 谭明谱^*

南京农业大学生命科学学院, 南京210095

通信作者：谭明谱；E-mail: tempo@njau.edu.cn；Tel: 025-84396372

摘　要：

植物在吸收和富集重金属能力上表现出显著的种内差异即基因型差异。本研究筛选了90份水稻核心种质, 测定50 μmol•L^-1镉(Cd)处理后的相对根长差异量(RRL)和相对株高差异量(RPH), 藉此确定Cd耐性较好的水稻品种有11个, Cd敏感的有9个。进一步检测在Cd污染土壤中生长的部分品种水稻各组织中的Cd及Ca、K、Mg、Fe、Mn、Zn等含量, 结果表明Cd影响了其他营养元素的积累, 并且表现出组织特异性和种质特异性。同其他材料相比, 仅耐Cd材料‘BINIRHEN’各组织Cd含量较低, 并且其穗中的Mn、Fe和Zn含量均大于茎, 因此‘BINIRHEN’为Cd胁迫遗传改良的优势材料。本研究结果对筛选Cd耐受的水稻品种以及克隆水稻基因组中耐受Cd的关键基因提供参考。

关键词：水稻; 核心种质; 镉; 耐性

收稿：2015-08-03   修定：2015-10-09

资助：国家自然科学基金(31271421)。

Identification of Cadmium Tolerance in a Subset of the Core Collection of Chinese Rice (Oryza sativa L.) Germplasm

JIANG Miao, YU Hai-Juan, LI Liang, TAN Ming-Pu^*

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China

Corresponding author: TAN Ming-Pu; E-mail: tempo@njau.edu.cn; Tel: 025-84396372

Abstract:

Plant uptake and accumulation of heavy metals show significant intraspecific difference namely genotypic differences. In this study, the relative difference of root length (RRL) and the relative difference of plant height (RPH) of 90 core collection of rice seedlings after 50 μmol•L^-1 cadmium (Cd) treatment were analyzed to determine the Cd tolerance of different rice materials. The results show that 11 cultivars display better resistance to Cd stress, whereas 9 lines sensitive to Cd treatment. Next, the contents of Cd, Ca, Fe, Zn, K, Mn and Mg were detected in the different tissues of rice grown in Cd-contaminated soil, and the results indicate that Cd affect the absorption of other beneficial elements. Furthermore, the distribution of the metal elements displayed tissue-specific. Compared with other materials, the Cd content of ‘BINIRHEN’ was remarkably lower, and the content of Zn, Fe and Mn in the spike was higher than that in the stem. These results suggest that ‘BINIRHEN’ was the most promising material for genetic improvement of Cd stress. The results of this study have certain reference value for the screening of Cd tolerant rice lines, and for cloning key genes controlling Cd tolerance in rice.

Key words: rice; core collection; cadmium; tolerance